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| Munari,F.M.; De-Paris,F.; Salton,G.D.; Lora,P.S; Giovanella,P; Machado,A.B.M.P; Laybauer,L.S.; Oliveira,K.R.P.; Ferri,C; Silveira,J.L.S.; Laurino,C.C.F.C; Xavier,R.M.; Barth,A.L; Echeverrigaray,S; Laurino,J.P.. |
| Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at ≥36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus agalactiae; Group B Streptococcus; Atr gene; Screening test; Accuracy. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100029 |
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| d'Azevedo,Pedro A.; Gonçalves,Ana Lúcia S.; Musskopf,Maiara I.; Ramos,Carina G.; Dias,Cícero A. G.. |
| Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67%) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54%) isolates were confirmed as ESBL producers, and 18/107 (17%) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83%) of strains that did not show CAIE. ESBL detection... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESBL; Double disk confirmatory test; Cefoxitin susceptibility testing; Screening test. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500006 |
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